Antibacterial effect of hydroethanolic Annona muricata leaf extract against Streptococcus sanguinis ATCC 10556: an in vitro study
DOI:
https://doi.org/10.64716/yj2dbd62Palabras clave:
Annona muricata., Antibacterial Agents, Plant Extracts, Streptococcus sanguinis, ChlorhexidineResumen
Introduction: The increasing interest in plant-derived antimicrobial compounds has encouraged the evaluation of Annona muricata as a potential source of bioactive substances against oral microorganisms. Streptococcus sanguinis is an early colonizer of dental biofilm and may participate in the establishment of complex oral microbial communities. Objective: To evaluate the in vitro antibacterial activity of three concentrations of hydroethanolic Annona muricata leaf extract against Streptococcus sanguinis ATCC 10556. Methods: An experimental in vitro study was conducted using hydroethanolic leaf extract at concentrations of 50%, 75%, and 100%, with eight replicates per group. Chlorhexidine 0.12% and ethanol 70% were used as positive and negative controls, respectively. Antibacterial activity was evaluated by agar disk diffusion, and inhibition-zone diameters were measured in millimeters. Differences among groups were assessed using the Kruskal–Wallis test. Results: Mean inhibition-zone diameters were 6.63 ± 0.52 mm, 13.06 ± 0.30 mm, and 16.44 ± 0.37 mm for the 50%, 75%, and 100% extracts, respectively. Chlorhexidine produced the largest inhibition zone (21.55 ± 1.23 mm), whereas ethanol produced 6.00 ± 0.00 mm. Overall differences among groups were statistically significant (p < 0.001). Conclusions: Hydroethanolic Annona muricata leaf extract exhibited concentration-dependent in vitro antibacterial activity against S. sanguinis ATCC 10556, with the greatest activity observed at 100%, although chlorhexidine showed the highest inhibitory effect.
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The data generated and analyzed during this study are available from the corresponding author upon reasonable request.
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Derechos de autor 2026 Alan Patricio Isique-Quesquen, Ruth Nerina Domínguez-Aburto (Autor/a)
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
Authors retain the copyright of their work and grant TUMI Oral Health the right of first publication. Articles are published under the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
